Identification of inhibitors targeting the energy-coupling factor (ECF) transporters

Journal article
Lipid membranes
Proteins
Drug design
Small molecules
Author

Eleonora Diamanti, Paulo C. T. Souza, Inda Setyawati, Spyridon Bousis, Leticia Monjas, Lotteke J.Y.M. Swier, Atanaz Shams, Aleksei Tsarenko, Weronika K. Stanek, Manuel Jäger, Siewert J. Marrink, Dirk J. Slotboom & Anna K. H. Hirsch

Doi

https://doi.org/10.1038/s42003-023-05555-x

Citation (APA 7)

Diamanti, E., Souza, P. C., Setyawati, I., Bousis, S., Monjas, L., Swier, L. J., … & Hirsch, A. K. (2023). Identification of inhibitors targeting the energy-coupling factor (ECF) transporters. Communications biology, 6(1), 1182.

Abstract

The energy-coupling factor (ECF) transporters are a family of transmembrane proteins involved in the uptake of vitamins in a wide range of bacteria. Inhibition of the activity of these proteins could reduce the viability of pathogens that depend on vitamin uptake. The central role of vitamin transport in the metabolism of bacteria and absence from humans make the ECF transporters an attractive target for inhibition with selective chemical probes. Here, we report on the identification of a promising class of inhibitors of the ECF transporters. We used coarse-grained molecular dynamics simulations on Lactobacillus delbrueckii ECF-FolT2 and ECF-PanT to profile the binding mode and mechanism of inhibition of this novel chemotype. The results corroborate the postulated mechanism of transport and pave the way for further drug-discovery efforts.